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Nucleic Acids Research, 1989, Vol. 17, No. 12 4567-4577
© 1989

MOLECULAR BIOLOGY

Regions at the carboxyl end of bacteriophage ø29 protein p6 required for DNA binding and activity in ø29 DNA replication

María J. Otero and Margarita Salas

Centro de Biología. Molecular (CSIC-UAM), Universidad Autónoma Canto Blanco, 28049 Madrid, Spain

Received March 17, 1989. Revised May 17, 1989. Accepted May 17, 1989.

Series of deletions corresponding to the carboxyl end of the phage ø29 protein p6 have been constructed and their activity in the initiation of ø29 DNA replication and their capacity to interact with the ø29 DNA ends have been studied. Determination of the activity of the deletion mutants in ø29 DNA replication indicated the dispensability of the 14 carboxy–terminal amino acids of the protein. The activity of protein p6 decreased with deletions from 23 to 39 amino acids and was undetectable when 44 amino acids were removed. A similar behaviour was obtained when the interaction of the mutant proteins with the ø29 DNA ends was analyzed. These results indicate that the stimulation of ø29 DNA replication by protein p6 requires a specific binding to the ø29 DNA ends.


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