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Nucleic Acids Research, 1989, Vol. 17, No. 12 4745-4755
© 1989

MOLECULAR BIOLOGY

Determinant differences between the rabbit and mouse immunoglobulin x enhancers impair the activity of the rabbit enhancer in mouse myeloma cells

Marie-André Akimenko, Marc Ekker, Noëlle Doyen, Christine Biben and Francçois Rougeon

Unité de Génétique et Biochimie du Développement, LACNRS 361, Département d'Immunologie, Institut Pasteur 25 rue du Dr Roux, 75724 Paris Cedex 15, France

Received March 23, 1989. Accepted May 5, 1989.

Enhancer activity of the rabbit immunoglobulin kappa light chain gene intron conserved region (KICR) was examined in mouse myeloma cells using transient expression experiments. Compared to the homologous region of the mouse {kappa} light chain gene, the rabbit KICR shows nearly no stimulatory effect on expression of the indicator gene, cat. Experiments with mouse-rabbit chimeric KICRs indicated that differences in the region around the NF-{kappa}B binding site are responsible for the impaired activity of the rabbit KICR whereas mouse sequences covering the {kappa}E2 and {kappa}E3 motifs can be replaced by the equivalent rabbit fragment without affecting enhancer function. Creation of a perfect mouse NF-{kappa}B target sequence in the rabbit gene only partially restores enhancer activity. Furthermore, mouse and rabbit DNA fragments encompassing the NF-{kappa}B target sequence behave in an identical manner in an electrophoretic mobility shift assay. The results indicate species-related functional differences in the immunoglobulin {kappa} light chain gene enhancer and suggest that although the NF-{kappa}B binding site plays a crucial role in enhancer activity surrounding gene elements are also necessary for full enhancer effect.


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