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Nucleic Acids Research, 1989, Vol. 17, No. 13 4917-4924
© 1989


MOLECULAR BIOLOGY

An ARS/silencer binding factor also activates two ribosomal protein genes in yeast

J.C. Dorsman, M.M. Doorenbosch1, C.T.C. Maurer1, J.H. de Winde, W.H. Mager1, R.J. Planta1 and L.A. Grivell

Section for Molecular Biology, Department of Molecular Cell Biology, University of Amsterdam Amsterdam, The Netherlands 1Laboratory of Biochemistry, Vrije Universiteit Amsterdam, The Netherlands

Received May 21, 1989. Accepted June 7, 1989.

GFI is an abundant yeast DNA-binding protein, capable of binding to both ARS sequences and to the upstream regions of a number of nuclear genes coding for mitochondria] proteins (Dorsman et al., Nucl. Acids Res., 16 [1988] 7287—7301). GFI binding sites conform to the consensus RTCRYN5ACG, an element also present in the binding sites of factors designated SUF and TAF. These factors act as trans-activators of the constitutive transcription of the genes for ribosomal proteins S33 and L3 respectively. We now present evidence that GFI, TAF and SUF are probably the same protein. We speculate that one of the functions of GFI is the adjustment of the expression of a number of gene families to cell growth rate.


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