Skip Navigation

This Article
Right arrow Print PDF (2318K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (55)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Gagnor, C.
Right arrow Articles by Leblcu, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gagnor, C.
Right arrow Articles by Leblcu, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 13 5107-5115
© 1989

MOLECULAR BIOLOGY

{alpha}-DNA IX: parallel annealing of {alpha}-anomeric oligodeoxyribonucleotides to natural mRNA is required for interference in RNase H mediated hydrolysis and reverse transcription

Corinne Gagnor, Bernard Rayner1, Jean-Paul Leonetti, Jean-Louis Imbach1 and Bernard Leblcu

Laboratoire de Biochimk des Proteines UA 1191 CNRS 1Laboratoire de Chimie Bio-Organique UA 488 CNRS University des Sciences et Techniques du Languedoc, Place E. Bataillon 34060 Montpellier CWex 1, France

Received March 16, 1989. Revised June 7, 1989. Accepted June 7, 1989.

ps- and aps-{alpha} anomeric oligodeoxyribonucleotides were designed to recognize in parallel (ps) or antiparallel (aps) orientation two different sites of a 1000 base-long rnRNA. Northern blots experiments indicate that only ps-{alpha}-oligonucleotides were able to hybridize to the mRNA target. Furthermore, only ps-a-oligonucleotides were able, in a sequence specific way, to protect the mRNA target against RNase H mediated hydrolysis or to inactivate the priming capacity of ß-oligodeoxynucleotide probes in reverse transcription. Formation of parallel-stranded mRNA {alpha}-oligonucleotide miniduplexes which prevents hybridization of ß-oligonucleotide probes is the most likely mechanism accounting for these results. Use of a-oligonucleotides as potential gene control agents is discussed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.