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Nucleic Acids Research, 1989, Vol. 17, No. 13 5115-5124
© 1989

MOLECULAR BIOLOGY

Chemiluminescent detection of DNA: application for DNA sequencing and hybridization

Stephen Beck*, Thomas O'Keeffe, James M. Coull and Hubert Koster

MilliGen/Biosearch, EHvision of Millipore 186 Middlesex Turnpike, Burlington, MA 01803, USA

*To whom corresspondance to be addressed

Received March 14, 1989. Revised . Accepted .

A non-radioactive DNA detection chemistry is described and its application is shown for DNA hybridization and standard dideoxy DNA sequencing. The method employes a biotin-streptavidin system which binds an enzyme specifically to a target DNA and upon exposure to substrate, the enzyme catalyzes a chemiluminescent reaction. The image is captured within seconds by a Polaroid or X-ray film. The method is capable of detecting DNA in the hundred attomol range.


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