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Nucleic Acids Research, 1989, Vol. 17, No. 13 5163-5172
© 1989

MOLECULAR BIOLOGY

Construction of a 42 base pair double stranded DNA microcircle

Mark Wolters* and Burghardt Wittig

Institut fär Molelcularbiologie und Biochemie, Freie Universitüt Berlin D-1000 Berlin 33, Amimallee 22, FRG

*To whom correspondence should be addressed

Received March 2, 1989. Revised May 25, 1989. Accepted May 25, 1989.

A procedure for the construction of double stranded DNA microcircles is described that overcomes the natural limits of established circularization procedures. Starting with two synthetic oligonucleotides which are able to form dumbbell shaped structures, two subsequent ligation reactions yield a microcircle of double stranded DNA of 42 base pairs. This is by far the smallest circle of double stranded DNA yet described. These microcircles can be constructed in quantities required for high resolution structural analyses such as X-ray crystallography and NMR spectroscopy.


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