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Nucleic Acids Research, 1989, Vol. 17, No. 13 5259-5272
© 1989

MOLECULAR BIOLOGY

Isolation and functional analysis of chicken 90-kDa heat shock protein gene promoter

Claire Vourc'h, Nadine Binart, Béatrice Chambraud, Jean-Pierre David, Valérie Jér{circ}me, Etienne-Emile Baulieu and Maria-Grazia Catelli

INSERM U33, Laboratoire Hormones 94275 Bicére Cédex, France

Received March 30, 1989. Revised May 30, 1989. Accepted May 30, 1989.

We report the nucleotide sequence of a 2652 bp derived from a chicken 90kDa heat shock protein (hsp 90) genomic clone. This fragment contains 890 bp ofthe 5' flanking region and 1762 bp of structural gene sequence encoding the first 85 amino acids of the protein. The start site of transcription was determined byprimer extension and RNase mapping. Two introns have been identified. The firstintron presents two features in common with the unique intron of the hsp 83 ofdrosophila: its location just before the ATG initiation codon and its length of 1.3 Kb. The 5' flanking region contains a TATAA element, a CCAAT box andseveral putative cis-regulatory elements that might account for the basal level ofexpression and developmental regulation of the gene. Functional analyses showthat hsp 90 gene expression is constitutive and heat inducible and that a full heatshock response requires the cooperativity of two distinct blocks of overlappingheat shock response elements.


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