Skip Navigation

This Article
Right arrow Print PDF (1060K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hotoda, H.
Right arrow Articles by Hata, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hotoda, H.
Right arrow Articles by Hata, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 13 5291-5306
© 1989

MOLECULAR BIOLOGY

Pre–activation strategy for oligodeoxyribonudeotide synthesis using triaryloxydichloro–phosphoranes in the phosphotriester method

Hitoshi Hotoda, Takeshi Wada, Mitsuo Sekine and Tsujiaki Hata*

Department of Life Chemistry, Tokyo Institute of Technology Nagatsuta, Midoriku, Yokohama 227, Japan

*To whom correspondence should be addressed

Received March 22, 1989. Revised May 30, 1989. Accepted May 30, 1989.

Triaryloxydlchlorophosphoranes were tested as condensing agents for Oliodeoxyribonudeotide synthesis in the phosphotriester method. Tris(2,4,6–tribromophenoxy)dlchlorophosphorane (BDCP) was found to be a relatively stable crystalline material which could be used as a chemical reagent. A notable feature of the BDCP-promoted condensation reaction was studied by a small amount of BDCP compared to the conventional condensing agent was effective for the generation of active nucleotide intermediates and BDCP itself was quantitatively converted into an inert material, tris(2,4,6-tribromophenyl)phosphate (2)– Thus, BDCP enabled us to separate the activation step from the condensation process in the phosphotriesterm ethod. This preactivation method was applied to the solid-phase synthesis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.