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Nucleic Acids Research, 1989, Vol. 17, No. 13 5339-5348
© 1989

MOLECULAR BIOLOGY

DNase I sensitivity of immunoglobulin light chain genes in Abelson murine leukemia virus transformed pre-B cell lines

Denise M. Persiani and Erik Selsing1

Program in Biophysics, Brandeis University, Waltham, MA 02254, USA 1Department of Biology and Rosenstiel Basic Medical Sciences Research Center Brandeis University, Waltham, MA 02254, USA

Received January 12, 1989. Revised May 30, 1989. Accepted May 30, 1989.

We have used Abelson murine leukemia virus (A-MuLV) transformed pre-B cell lines to test the hypothesis that the rearrangement potential of a developing B-lymphocyte is dependent on an opening of the chroraatin structure surrounding immunoglobulin (Ig) genes, thus allowing accessibility to an Ig gene recombinase.The chromatin structures surrounding heavy (H), kappa (K), and lambda ({lambda}) chain constant-region genes were assessed by DNase I sensitivity in A-HuLV transformed cell lines capable of H, K or {lambda} gene rearrangement. Our results indicate that DNase I-sensitive chromatin structures of these Ig constant-region genes correlate closely with the ability of the genes to undergo recombination. We also find that the chromatin structure of an Ig constantregion locus becomes DNase I sensitive before any DNA rearrangement events occur.


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