Skip Navigation

This Article
Right arrow Print PDF (2034K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (65)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Murakawa, G. J.
Right arrow Articles by Sigman, D. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murakawa, G. J.
Right arrow Articles by Sigman, D. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 13 5361-5376
© 1989

MOLECULAR BIOLOGY

Scission of RNA by the chemical nuclease of 1,10-phenanthroline-eopper ion: preference for single-stranded loops

George J. Murakawa, Chi-hong B. Chen1, Michio D. Kuwabara1, Donald P. Nierlich* and David S. Sigman1,*

1Departments of Microbiology and Biological Chemistry, Molecular Biology Institute, University of California Los Angeles, CA 90024, USA

*To whom correspondence should be addressed

Received October 17, 1988. Revised June 5, 1989. Accepted June 5, 1989.

The scission of RNA by the chemical nuclease activity of 1,10-phenanthroline-copper (OP-Cu) has been studied using a lac mRNA fragment and tRNAPhe as substrates. Since the chemical mechanism of scission involves oxidative attack on the ribose, scission is observed at all nucleotides including dihydrouridine and Y-bases. Specificity for single-stranded loop regions is apparent from the similarity of the reactivity of OP-Cu to the single-strand specific reagents dimethyl sulfate and diethyl pyrocarbonate using the fragment of lac mRNA as a substrate. Similar preference is observed in the reaction with tRNA although scission in the helical acceptor stem is also observed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
R. Erlitzki, J. C. Long, and E. C. Theil
Multiple, Conserved Iron-responsive Elements in the 3'-Untranslated Region of Transferrin Receptor mRNA Enhance Binding of Iron Regulatory Protein 2
J. Biol. Chem., November 1, 2002; 277(45): 42579 - 42587.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Shomron, H. Malca, I. Vig, and G. Ast
Reversible inhibition of the second step of splicing suggests a possible role of zinc in the second step of splicing
Nucleic Acids Res., October 1, 2002; 30(19): 4127 - 4137.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Ke and E. C. Theil
An mRNA Loop/Bulge in the Ferritin Iron-responsive Element Forms in Vivo and Was Detected by Radical Probing with Cu-1,10-phenantholine and Iron Regulatory Protein Footprinting
J. Biol. Chem., January 18, 2002; 277(4): 2373 - 2376.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.