Nucleic Acids Research, 1989, Vol. 17, No. 14 5427-5446
© 1989
MOLECULAR BIOLOGY |
Kinetoplastid mitochondria contain functional tRNAs which are encoded in nuclear DNA and also contain small mlnltircJe and maxicircle transcripts of unknown function
Department of Biology and Molecular Biology Institute, University of California Los Angeles, CA 90024 1Department of Biology, University of Pennsylvania Philadelphia, PA 19104 2Department of Microbiology and Immunology, University of California School of Medicine, University of California Los Angeles, CA 90024, USA 3Department of Biochemistry, Federal University of Rio Grande do Sul,Porto Alegre Brazil
Received June 6, 1989. Accepted June 14, 1989.
The mitochondrion of Leishmania tarentolae contains approximately 35—40 tRNAs, many of which comigrate with cytoplasmic tRNAs. Both mitochondrial (KtRNA) and cytoplasmic (CtRNA) tRNAs are functional, as they could be acylated either by mitochondrial or cytoplasmic synthetase extracts. There are two methionyl tRNA species in the cytoplasmic and mitochondrial fractions, one of which is unique to each fraction, indicating that the KtRNA fraction is free of CtRNA contamination. Leucyl and glycyl tRNAs were identified by hybridization with a genomic clone from Trypanosoma brucei. KtRNA hybridizes with nuclear chromosomes, but not with minicircle or maxicircle DNA. KtRNA isolated by DEAE chromatography or agarose gel electrophoresis contains additional small RNAs which hybridize with both minicircle and maxicircle DNA. These transcripts do not migrate like tRNAs in acrylamide gels and their function is unknown. We suggest that most if not all mitochondrial tRNAs in L tarentolae are nuclear-encoded and imported into the mitochondrion.
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