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Nucleic Acids Research, 1989, Vol. 17, No. 14 5501-5508
© 1989

MOLECULAR BIOLOGY

Translational reinitiation in the presence and absence of a Shine and Dalgarno sequence

Remco A. Spanjaard and Jan van Duin*

Department of Biochemistry, Gorlaeus Laboratories, University of Leiden Einsteinweg 5, 2333 CC Leiden, The Netherlands

*To whom correspondence should be addressed

Received May 3, 1989. Revised June 26, 1989. Accepted June 26, 1989.

The process of translational reinitiation in Escherichia coli was studied in a two cistron system where expression of the downstream reporter gene was dependent on translation of an upstream reading frame. The dependence was almost absolute. Upstream translation increased expression of the downstream gene by two to three orders of magnitude. This large difference aJlowed us to quantitate restarts in a meaningful manner. In the absence of a Shine and Dalgarno (SD) region reinitiationoccurred but its efficiency was about 10% of that found in the SD carrying counterpart. We discuss three ways by which translational coupling between neighboring cistrons can be enforced.


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