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Nucleic Acids Research, 1989, Vol. 17, No. 14 5529-5536
© 1989

MOLECULAR BIOLOGY

Mechanism of incision by an apurinic≤apyrimidinjc endonuclease present in human placenta

Bjam-Ivar Haukanes*, Christian-Urs Wittwer1 and Dag Emil Helland

Laboratory of Biotechnology, University of Bergen, Bergen 9001 Tromse, Norway 1Department of Biochemistry, Institute of Medical Biology University of Tromse 9001 Tromse, Norway

*To whom correspondence should be addressed

Received April 24, 1989. Revised June 12, 1989. Accepted June 12, 1989.

An apparently homogeneous enzyme preparation, containing an apurinic/apyrimidinic (AP) endonuclease from human placenta, was by DNA sequencing analysis found to act as a class I AP-endonuclease, i.e. produce a 3'-deoxyribose and 5'-phosphomonoester nucleotide termini.


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