Skip Navigation

This Article
Right arrow Print PDF (3701K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Sehgal, A.
Right arrow Articles by Chao, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sehgal, A.
Right arrow Articles by Chao, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 14 5623
© 1989

MOLECULAR BIOLOGY

Gene transfer of truncated NGF receptor clones leads to cell surface expression in mouse fibroblasts

Amita Sehgal, Bothwell Mark1 and Moses Chao

Department of Cell Biology and Anatomy, Cornell University Medical College New York, NY 10021 1Department of Physiology and Biophysics, University of Washington School of Medicine Seattle, WA 98195, USA

Received February 1, 1989. Revised May 10, 1989. Accepted May 10, 1989.

Transfection of recombinant bacteriophage clones encoding human NGF receptor sequences resulted in cell surface expression in mouse fibroblasts. Unexpectedly, receptors were expressed even after transfection with phage clones which lack 5’ gene sequences. Stable transformants were purified and analyzed in detail. SI nuclease protection and primer extension analysis revealed that an initiation site lies within an intron sequence in the middle of the receptor gene. A truncated mRNA transcript was detected that allowed for the expression of NGF receptors capable of binding to NGF. Since the original phage clones lacked the first two exons, these results suggest that the normal N-terminal sequences may not be necessary for cell surface expression and binding to NGF.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.