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Nucleic Acids Research, 1989, Vol. 17, No. 14 5651-5664
© 1989

MOLECULAR BIOLOGY

Transcription of the chicken myb proto-oncogene starts within a CpG island

Michal Dvo{lambda}aék, Pavel Urbànek, Petr Bart{upsilon}uèk, Vaéclav Paces, Jaromir Vlach1, Vladimir Peccnka, Lubo{sigma} Arnold1, Miloslav Tràvnfcek and Josef {rho}iman

Institute of Molecular Genetics 1Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences Flemingovo nam. 2, 166 37 Prague 6, Czechoslovakia

Received June 8, 1989. Revised June 27, 1989. Accepted June 27, 1989.

The nucleotide sequence of an 8.2–kb DNA fragment from the 5' proximal part of the chicken myb proto–oncogene spanning 1761 nucleotides upstream and 6436 nucleotides downstream from a presumed c–myb initiation codon was determined. A 33–kb G+ C–rich region found in this sequence had also other features characterizing CpG islands,i.e. no CpG underrepresentation and lack of CpG methylation. In haematopoietic tissues –myb mRNA synthesis starts in two major regions of the CpG island, namely 98 to 108 and 143 to 145 nucleotides upstream from the c–myb initiation codon. These two regions are in or close to the 124–bp evolutionarily conserved element located in the middle part of the CpG island. No alternative splicing of the 5' end of c–myb mRNA suggested earlier(1,2) was observed. The c–myb promoter contains neither TATA nor CAAT box–like structures at the usual positions. Instead, numerous potential Sp1 factor binding sites were found both upstream and downstream from the transcription initiation sites. Moreover, consensus v–myb protein DNA–binding sites were revealed in the promoter region and in sequences downstream from it.


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