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Nucleic Acids Research, 1989, Vol. 17, No. 14 5679-5685
© 1989

MOLECULAR BIOLOGY

Mutagenesis analysis of a self-cleaving RNA

Candice C. Sheldon and Robert H. Symons*

Department of Biochemistry, University of Adelaide Adelaide, SA 5000, Australia

*To whom correspondence should be addressed

Received May 17, 1989. Revised June 7, 1989. Accepted June 7, 1989.

The hammerhead structural model proposed for sequences that mediate self-cleavage of certain RNAs contains base-paired three stems and 13 conserved bases. Insertion, deletion and base substitution mutations were carried out on a 58 base RNA containing the sequence of the single-hammerhead structure of the plus RNA of the virusoid of lucerne transient streak virus,and the effects on self-cleavage assessed. Results showed that there is flexibility in the sequence requirements for self-cleavage in vitro, but alterations of the conserved sequence or predicted secondary structure generally reduced the efficiency of self-cleavage.


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