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Nucleic Acids Research, 1989, Vol. 17, No. 14 5793
© 1989

MOLECULAR BIOLOGY

Differential replication of circular DNA molecules co-injected into early Xenopus Laevis embryos

Nicholas J. Marini1,2, K.T. Hiriyanna1 and Robert M. Benbow1,*

1Department of Zoology, Lowa State university Ames, IA 50011 2Department of Biology, The Johns Hopkins University Baltimore, MD 21218, USA

*To whom correspondence should be addressed

Received February 27, 1988. Revised June 5, 1989. Accepted June 5, 1989.

Replication of co-injected supercoiled DNA molecules in fertilized Xenopus eggs was monitored through the blastula stage of development. The extent of replication, as measured by 32p-dTMP Incorporation Into form I DNA, was directly proportional to the number of molecules, rather than the size, of the plasmid injected. Although only a small fraction of molecules of either template was replicated, incorporation was predominantly into full length daughter molecules. Over at least a 20-fold concentration range of microinjected DNA, injection of equal masses of DNA resulted In greater incorporation into the smaller form I DNA present in molar excess. The extent of incorporation into supercoiled DNA for a particular plasmid was apparently independent of the concentration of a second, co-injected plasmid. The relative extents of replication of co-injected supercoiled templates could be altered simply by changing the molar ratios of the templates.


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