Nucleic Acids Research, 1989, Vol. 17, No. 15 5923-5931
© 1989
MOLECULAR BIOLOGY |
Altered mRNA cap recognition activity of initiation factor 4E in the yeast cell cyde division mutant cdc33
Institut für Biochemie und Molekularbiologie der Universität Bern Bühlstrasse 28, CH-3012 Bern, Switzerland
*To whom correspondence should be addressed
Received May 30, 1989. Accepted June 29, 1989.
The mutation in the S.cerevisiae cell cycle division mutant cdc33 consists of a single G to A transition in the open reading frame encoding translation initiation factor 4E (eIF-4E). This leads to the substitution of glycine113 by aspartic acid close to tryptophane 115 in the protein. This mutation reduces cap binding activity of eUM-4E as measured by binding of eIF-4E to m7GDP agarose columns and slows down overall protein synthesis at the non-permissive temperature. Comparison of the cdc33 mutation with other mutations affecting eIF-4E function supports the view that tryptophane residues and their flanking regions are involved in cap binding activity of eIF-4E.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Ohn, Y.-C. Chiang, D. J. Lee, G. Yao, C. Zhang, and C. L. Denis CAF1 plays an important role in mRNA deadenylation separate from its contact to CCR4 Nucleic Acids Res., May 14, 2007; 35(9): 3002 - 3015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Rosenfeld and V. R. Racaniello Hepatitis C Virus Internal Ribosome Entry Site-Dependent Translation in Saccharomyces cerevisiae Is Independent of Polypyrimidine Tract-Binding Protein, Poly(rC)-Binding Protein 2, and La Protein J. Virol., August 15, 2005; 79(16): 10126 - 10137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. HERNANDEZ, P. VAZQUEZ-PIANZOLA, J. M. SIERRA, and R. RIVERA-POMAR Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos RNA, November 18, 2004; 10(11): 1783 - 1797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lynch, C. Fitzgerald, K. A. Johnston, S. Wang, and E. V. Schmidt Activated eIF4E-binding Protein Slows G1 Progression and Blocks Transformation by c-myc without Inhibiting Cell Growth J. Biol. Chem., January 30, 2004; 279(5): 3327 - 3339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Léonard, D. Plante, S. Wittmann, N. Daigneault, M. G. Fortin, and J.-F. Laliberté Complex Formation between Potyvirus VPg and Translation Eukaryotic Initiation Factor 4E Correlates with Virus Infectivity J. Virol., September 1, 2000; 74(17): 7730 - 7737. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. T. Brown, X. Yang, and A. W. Johnson Inhibition of mRNA Turnover in Yeast by an xrn1 Mutation Enhances the Requirement for eIF4E Binding to eIF4G and for Proper Capping of Transcripts by Ceg1p Genetics, May 1, 2000; 155(1): 31 - 42. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. L. Neff and A. B. Sachs Eukaryotic Translation Initiation Factors 4G and 4A from Saccharomyces cerevisiae Interact Physically and Functionally Mol. Cell. Biol., August 1, 1999; 19(8): 5557 - 5564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gao, W. Rychlik, and R. E. Rhoads Cloning and Characterization of Human eIF4E Genes J. Biol. Chem., February 20, 1998; 273(8): 4622 - 4628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. de la Cruz, I. Iost, D. Kressler, and P. Linder The p20 and Ded1 proteins have antagonistic roles in eIF4E-dependent translation in Saccharomyces cerevisiae PNAS, May 13, 1997; 94(10): 5201 - 5206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Winstall, M. Sadowski, U. Kuhn, E. Wahle, and A. B. Sachs The Saccharomyces cerevisiae RNA-binding Protein Rbp29 Functions in Cytoplasmic mRNA Metabolism J. Biol. Chem., July 14, 2000; 275(29): 21817 - 21826. [Abstract] [Full Text] [PDF]
|
|