Skip Navigation

This Article
Right arrow Print PDF (1604K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (42)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Mag, M.
Right arrow Articles by Engels, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mag, M.
Right arrow Articles by Engels, J. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 15 5973-5988
© 1989

CHEMISTRY

Synthesis and selective cleavage of oligodeoxyribonucleotldes containing non-chiral intemucieotlde phosphoramidate linkages

Matthias Mag and Joachim W. Engels

Institut für Orgamsche Chemie, Johann Wolfgang Goethe-Universitüt Frankfurt Niederurseler Hang, 6000 Frankfurt am Main 50, FRG

Received May 22, 1989. Accepted July 13, 1989.

Oligdeoxynucleotides containing phosphoramidote internucleotide links 3'-OP(O)NH-5' have been prepared using standard solid phase phosphoramidite techniques. For the incorporation of the phosphoramkkrte linkages we have used monomer as well as dimer building blocks. With die monomer 3'-phosphoramidite building blocks, which are derived from 5'-amino-2', 5'-dideoxynucleosides, it is possible to incorporate phosphoramidate links into specific positions widiin an oligodeoxynucleotide. Furthermore the synthesis of several dinucleoside phosphate derivatives which are linked by phosphoramidate bonds are described. The internucleotide phosphoramidate linkage was performed using die Staudinger reaction followed by a Michaelis-Arbuzov type transformation. After 3'-phosphitylation these dinucleosides are compatible with the current phosphoramidite methodology of oligodeoxynucleotide synthesis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. Narayanan, J. Gall, and C. Richert
Clamping down on weak terminal base pairs: oligonucleotides with molecular caps as fidelity-enhancing elements at the 5'- and 3'-terminal residues
Nucleic Acids Res., May 20, 2004; 32(9): 2901 - 2911.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. T. Pon and S. Yu
Linker phosphoramidite reagents for the attachment of the first nucleoside to underivatized solid-phase supports
Nucleic Acids Res., January 29, 2004; 32(2): 623 - 631.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. L. Wolfe, T. Kawate, A. Belenky, and V. Stanton Jr
Synthesis and polymerase incorporation of 5'-amino-2',5'-dideoxy-5'-N-triphosphate nucleotides
Nucleic Acids Res., September 1, 2002; 30(17): 3739 - 3747.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.