Nucleic Acids Research, 1989, Vol. 17, No. 15 6065-6075
© 1989
CHEMISTRY |
Synthesis and biological evaluation of some phosphate triester derivatives of the anti-viral drug AraA
Department of Chemistry, University College London 20 Gordon Street, London WC1 OAJ 1Department of Chemical Pathology, University College and Middlesex School of Medicine Windeyer Building, Cleveland Street, London W1P 6DB, UK
*To whom correspondence should be addressed
Received May 3, 1989. Revised July 4, 1989. Accepted July 4, 1989.
A number of novel phosphate triester derivatives of the anti-viral nucleoside analogue araA have been prepared by a rapid 2-step procedure, not necessitating prior sugar protection. Spectroscopic and lipophilicity data have been collected on these compounds, and they have been assayed with a range of hydrolytic enzymes. The compounds have been found to be highly resistant to hydrolysis at physiological pH, enzymatic or otherwise. An in vitro assay indicated inhibition of DNA synthesis by mammalian cells, by each of these compounds, in the range 3–300µM. Moreover, the degree of inhibition showed a close correlation to chemical structure; in particular, there was a direct relationship between inhibition of thymidine incorporation and lipophilicity. These results suggest cellular penetration by the phosphate triesters and intracellular hydrolysis, by an unspecified mechanism, to the free nucleotide or nucleoside.