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Nucleic Acids Research, 1989, Vol. 17, No. 15 6129-6141
© 1989

CHEMISTRY

Uncharged stereoregular nucleic acid analogs: 2. Morpholino nucleoade otigomers with carbamate internucleoside linkages

Eugene P. Stirchak*, James E. Summerton and Dwight D. Weller1

Antivirals, Inc., 249 S.W. Avery Avenue, Corvallis, OR 97333 1Department of Chemistry, Oregon State University Corvallis, OR 97331, USA

*To whom correspondence should be addressed

Received April 4, 1989. Revised June 29, 1989. Accepted June 29, 1989.

A number of novel phosphate triester derivatives of the anti-viral nucleoside analogue araA have been prepared by a rapid 2-step procedure, not necessitating prior sugar protection. Spectroscopic and lipophilicity data have been collected on these compounds, and they have been assayed with a range of hydrolytic enzymes. The compounds have been found to be highly resistant to hydrolysis at physiological pH, enzymatic or otherwise. An in vitro assay indicated inhibition of DNA synthesis by mammalian cells, by each of these compounds, in the range 3–300µM. Moreover, the degree of inhibition showed a close correlation to chemical structure; in particular, there was a direct relationship between inhibition of thymidine incorporation and lipophilicity. These results suggest cellular penetration by the phosphate triesters and intracellular hydrolysis, by an unspecified mechanism, to the free nucleotide or nucleoside.


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