Skip Navigation

This Article
Right arrow Print PDF (4386K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Taccioli, G. E.
Right arrow Articles by Judewiez, N. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Taccioli, G. E.
Right arrow Articles by Judewiez, N. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 15 6153-6165
© 1989

MOLECULAR BIOLOGY

Ubiquitln expression in Neurospora crassa: cloning and sequencing of a polyubiquitin gene

Guillermo E. Taccioli, Erich Grotewold, Gabriel O. Aisemberg and Norberto D. Judewiez*

Institute de Investigaciones en Ingeniería Genética y Biologia Molecular (INGEBI) Vuelta de Obligado 2490 (1428), Buenos Aires, Argentina

*To whom correspondence should be addressed

Received March 23, 1989. Revised July 3, 1989. Accepted July 3, 1989.

We have cloned and sequenced a polyubiquitin gene from Neurospora crassa that is organized in a four repeat-tandem array. The first repeat contains a small intron and the last is fused to an extra glutamine codon. In Northern blots, two RNA species of 1.3 kb and 0.7 kb hybridize to the isolated clone. The larger ubiquitin (UBI) transcript accumulates after partial inhibition of protein synthesis with cycloheximide, and the smaller one preferentially accumulates in conidia after germination. Unexpectedly, constitutive expression of UBI transcripts in exponentially grown mycelia is not altered by heat-shock or exposure to arsenite.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
R. Kano, K. Okabayashi, Y. Nakamura, S. Watanabe, and A. Hasegawa
Expression of Ubiquitin Gene in Microsporum canis and Trichophyton mentagrophytes Cultured with Fluconazole
Antimicrob. Agents Chemother., September 1, 2001; 45(9): 2559 - 2562.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.