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Nucleic Acids Research, 1989, Vol. 17, No. 15 6241-6251
© 1989

MOLECULAR BIOLOGY

Cloning and characterization of mutL and mutS genes of Vibrio cholerae: nucleotide sequence of the mutL gene

Tapan Kumar Bera, Subrata K. Ghosh+ and Jyotimy Das*

Biophysics Division, Indian Institute of Chemical Biology 4 Raja S.C. Mullick Road, Calcutta 700032, India

*To whom correspondence should be addressed

Received May 24, 1989. Revised July 14, 1989. Accepted July 14, 1989.

The mutL and mutS genes of Vibrio cholerae have been identified using interspecific complementation of Escherichia coli mutL and mutS mutants with plasmids containing the gene bank of V. cholerae. The recombinant plasmid pJT470, containing a 4.7 kb fragment of V. cholerae DNA codes for a protein of molecular weight 92,000. The product of this gene reduces the spontaneous mutation frequency of the E. coli mutS mutant. The plasmid, designated pJT250, containing a 2.5 kb DNA fragment of V. cholerae and coding for a protein of molecular weight 62,000, complements the mutL gene function of E. coli mutL mutants. These gene products are involved in the repair of mismatches in DNA. The complete nucleotide sequence of mutL gene of V. cholerae has been determined.

+Present address: Department of Molecular Biology, The Cleveland Clinic Foundation, Cleveland, OH 441064775, USA


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