Nucleic Acids Research, 1989, Vol. 17, No. 16 6473-6484
© 1989
MOLECULAR BIOLOGY |
Identification of sequences regulating the transcription of a Dictyostclium gene selectively expressed in prespore cells
ICRF, Clare Hall Laboratory South Mimms, Herts EN6 3LD, UK
*Present address: National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 IAD, UK
Received June 23, 1989. Accepted July 28, 1989.
There has been considerable debate about the relative contributions of transcriptional and post-transcriptional mechanisms to the regulation of prespore gene expression in Dictyostelium. We have determined the DNA sequence upstream of D19, the Dictyostelium gene encoding PsA, a prespore-specific, cell surface protein of unknown function. Our analysis of gene fusions, in which D19 upstream sequences are placed adjacent to a heterologous reporter gene, indicates that transcriptional signals alone are sufficient for the correct temporal and cell-type specific expression of this gene. We also show that the 5' and 3' boundaries of the minimal sequences necessary for correct developmental regulation lie within the the region 338 to 122 nucleotides upstream of the start site of transcription but that flanking sequences seem to be necessary for optimal expression.
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