Skip Navigation

This Article
Right arrow Print PDF (2926K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Graessmann, M.
Right arrow Articles by Graessmann, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Graessmann, M.
Right arrow Articles by Graessmann, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 16 6603-6612
© 1989

MOLECULAR BIOLOGY

Helper activity for gene expression, a novel function of the SV40 enhancer

M. Graessmann, J. Menne, M. Liebler, I. Graeber and A. Graessmann

Institut für Molekularbiologie und Biochemie der Freien Universitat Berlin Amimallee 22, D-1000 Berlin 33, FRG

Received May 3, 1989. Revised July 19, 1989. Accepted July 19, 1989.

In this investigation we demonstrate that the enhancer of SV40 possesses an additional function which is a ‘helper activity’ for a more efficient transfer of viral DNA from the cytoplasm into the nucleus. After DNA transfection into rat-2 cells, the rate of CAT gene expression linked to SV40 promoter/enhancer (pSV2CAT) was approximately 50 fold higher than linked to the tk promoter (pBLCAT2). After direct nuclear microinjection this difference was reduced to a factor of 10. However cytoplasmic injection of the same number of DNA molecules/cell showed again a 50 fold increase for the SV40 promoter/enhancer-CAT construct. This difference was not due to selective degradation of the pBLCAT2 DNA. The ‘helper function’ did not require the intact 72 bp sequence. In vitro synthesized enhancers lacking certain enhancer motifs (GT-I, TC-II and TC-I sequence) were still effective after cytoplasmic injection whereas an 8 bp deletion (representing a part of the AP-I motif) on the downstream side strongly reduced the helper function after cytoplasmic injection but not the classical transcriptional enhancement after direct nuclear transfer.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Exp. Biol. Med.Home page
R. Zhou and D. A. Dean
Gene Transfer of Interleukin 10 to the Murine Cornea Using Electroporation
Experimental Biology and Medicine, March 1, 2007; 232(3): 362 - 369.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. L. Wilson, B. S. Dean, G. Wang, and D. A. Dean
Nuclear Import of Plasmid DNA in Digitonin-permeabilized Cells Requires Both Cytoplasmic Factors and Specific DNA Sequences
J. Biol. Chem., July 30, 1999; 274(31): 22025 - 22032.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
F. Langle-Rouault, V. Patzel, A. Benavente, M. Taillez, N. Silvestre, A. Bompard, G. Sczakiel, E. Jacobs, and K. Rittner
Up to 100-Fold Increase of Apparent Gene Expression in the Presence of Epstein-Barr Virus oriP Sequences and EBNA1: Implications of the Nuclear Import of Plasmids
J. Virol., July 1, 1998; 72(7): 6181 - 6185.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.