Skip Navigation

This Article
Right arrow Print PDF (3019K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (26)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Chrapkiewicz, N. B.
Right arrow Articles by Granner, D. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chrapkiewicz, N. B.
Right arrow Articles by Granner, D. K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 16 6651-6667
© 1989

MOLECULAR BIOLOGY

Rat gene 33: analysis of its structure, messenger RNA and basal promoter activity

Nancy B. Chrapkiewicz, Charles M. Davis, David T.-W. Chu, Catherine M. Caldwell and Daryl K. Granner

Department of Molecular Physiology and Biophysics, Vanderbilt University Medical School Nashville, TN 37232, USA

Received June 1, 1989. Revised July 21, 1989. Accepted July 21, 1989.

Several overlapping cDNA and genomic DNA clones corresponding to mRNAg33 and gene 33, respectively, vere isolated and characterized. The mRNAg33 molecule is 2971 nt in length, exclusive of a poly(A+) tail, and encodes a putative 459 anino acid protein (49,946 daltons). The 13.2 Icbp gene consists of four exons, three introns, and has two initiation sites located 27 and 30 bp downstream from a TATA box.

Transfection of H4IIE cells with a fusion gene 33 (–1900 to +32)/lucifer-ase construct (pSL330A) gave rise to readily detectable luciferase activity. In addition, primer extension analysis of the gene 33/luciferase dRNA transcribed in these experioents showed that transcription initiates in the gene 33 DNA at two sites consistent with those found in vivo. Analysis of the effect of 5' deletions on basal promoter activity showed that, in relation to the promoter activity of pSL330A, –40X of the activity is lost between –1643 and –1050, another –40X between –550 and –475, and deletion to –55 causes a total loss of detectable luciferase activity.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
R. K. Hall, X. L. Wang, L. George, S. R. Koch, and D. K. Granner
Insulin Represses Phosphoenolpyruvate Carboxykinase Gene Transcription by Causing the Rapid Disruption of an Active Transcription Complex: A Potential Epigenetic Effect
Mol. Endocrinol., February 1, 2007; 21(2): 550 - 563.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. Peshavaria, M. A. Cissell, E. Henderson, H. V. Petersen, and R. Stein
The PDX-1 Activation Domain Provides Specific Functions Necessary for Transcriptional Stimulation in Pancreatic {beta}-Cells
Mol. Endocrinol., December 1, 2000; 14(12): 1907 - 1917.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
L. Fiorentino, C. Pertica, M. Fiorini, C. Talora, M. Crescenzi, L. Castellani, S. Alemà, P. Benedetti, and O. Segatto
Inhibition of ErbB-2 Mitogenic and Transforming Activity by RALT, a Mitogen-Induced Signal Transducer Which Binds to the ErbB-2 Kinase Domain
Mol. Cell. Biol., October 15, 2000; 20(20): 7735 - 7750.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.