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Nucleic Acids Research, 1989, Vol. 17, No. 16 6669-6667
© 1989

CHEMISTRY

Conformations in crystals and solutions of d(CACGTG), d(CCGCGG) and d(GGCGCC) studied by vibrational spectroscopy

L. Urpi2, J.P. Ridoux1, J. Liquier, N. Verdaguer, I. Fita, J.A. Subirana, F. Iglesias2, T. Huynh-Dinh2, J. Igolen2 and E. Taillandier1

Unitat de Quimica Macromolecular, ETS Enginyers Industrials Diagonal 647 , 08028 Barcelona, Spain 1Laboratoire de Spectroscopie Biomoléculaire UFR Santé Métecine et Biologie Humaine 74 rue Marcel Cachin, 93012 Bobigny Cedex, France 2Unité de Chimie Organique, Institut Pasteur 28 rue du Dr Roux, 75724 Paris Cedex 15, France

Received April 26, 1989. Revised July 17, 1989. Accepted July 17, 1989.

Crystals of self complementary DNA hexamera d(CACGTG). d(CCGCGG) and d(GGCGCC) were grown by vapour diffusion technique and studied by microRaman and microIR epectroscoples. The oligonucleotldes were studied in parallel In solution by vibratlonal spectroscopy. A B->Z transition was detected by Raman spectroscopy during the crystallization process for d(CACGTG). Vibratlonal spectroscopy shows that the d(GGCGCC) crystals adopt a B geometry. On the contrary the d(CCGCGG) sequence which is shown to be able to undergo in solution or In films quite easily the B->2 transition, remains trapped in crystals in a geometry which may correspond to an intermediate conformation often proposed in models of the B->Z transition. The crystals used in this study were characterized by X-ray diffraction. The unit cell and space group have been determined.


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