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Nucleic Acids Research, 1989, Vol. 17, No. 16 6713-6719
© 1989

MOLECULAR BIOLOGY

A physical map of the genome of Ureapiasma urtalyticum 960T with ribosomal RNA loci

Benjamin G. Cocks, Louise E. Pyle and Lloyd R. Finch

Russell Grimwade School of Biochemistry, University of Melbourne Parlcville, Victoria 3052, Australia

Received April 7, 1989. Revised June 29, 1989. Accepted June 29, 1989.

A physical map is presented for the 900 kilobase pair genome of Ureaplasma urealyticum 960T, locating 29 sites for 6 restriction endonucleases. The large restriction fragments were separated and sized by pulsed-field agarose gel electrophoresis (PFGE). Their locations on the map were determined by probing Southern blots of digests with individual fragments isolated from other digests and by correlating the products of double digestions and partial digestions. An end-labelling technique was used to detect small fragments not readily observed by PFGE. Two loci for rRNA genes have been determined by probing with cloned DNA.


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