Skip Navigation

This Article
Right arrow Print PDF (1827K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (61)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Bowcock, A. M.
Right arrow Articles by Sehgal, P. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bowcock, A. M.
Right arrow Articles by Sehgal, P. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 17 6855-6864
© 1989

MOLECULAR BIOLOGY

Rapid detection and sequencing of alleles in the 3' flanking region of the Interleukin-6 gene

Anne M. Bowcock*, Anuradha Ray1, Henry Erlich2 and Pravinkumar B. Sehgal1

Department of Genetics, Stanford University Stanford, CA 94305 1Rockefeller University New York, NY 10021 2Cetus Corporation, Department of Human Genetics 1400 53rd Street, Emeryville, CA 94608, USA

*To whom correspondence should be addressed

Received June 28, 1989. Accepted July 27, 1989.

The 3' flanking region of the interleukin 6 gene is polymorphic due to insertions of different size. Within this region lies a sequence of approximately 500 base pairs that is AT rich. Based on flanking sequence information we have constructed oligonucleotides which prime the polymerase chain reaction (PCR) and amplify this AT rich region. The amplification products visualized by agarose gel electroplioresis gave fragment sizes for both homozygous and heterozygous individuals that were concordant with those observed by conventional genomic blotting techniques. Alleles that could not be typed by Southern analysis were resolved with this approach. These results illustrate the value of PCR for the rapid detection of length polymorphisms such as those due to variable numbers of tandem repeats. In contrast to RFLP analysis this procedure takes less than a day to perform, is cheaper, avoids the use of radioactivity and requires far less substrate DNA. Three different human alleles were sequenced, and differences were detected that were due to both large duplications and loss of one or two bases, suggesting that AT rich regions identify highly polymorphic loci. The same pruners also amplified non-human primate DNA, allowing a comparison of the human sequence with that of the common chimpanzee and baboon.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Bone Joint Surg BrHome page
P. V. Giannoudis, M. van Griensven, E. Tsiridis, and H. C. Pape
The genetic predisposition to adverse outcome after trauma
J Bone Joint Surg Br, October 1, 2007; 89-B(10): 1273 - 1279.
[Abstract] [Full Text] [PDF]

Home page
 TraumaHome page
C. C Tzioupis, S. Katsoulis, N. Manidakis, and P. V Giannoudis
The immuno-inflammatory response to trauma
Trauma, October 1, 2005; 7(4): 171 - 183.
[Abstract] [PDF]

Home page
 JBJSHome page
C. S. Roberts, H.-C. Pape, A. L. Jones, A. L. Malkani, J. L. Rodriguez, and P. V. Giannoudis
Damage Control Orthopaedics. Evolving Concepts in the Treatment of Patients Who Have Sustained Orthopaedic Trauma
J. Bone Joint Surg. Am., February 1, 2005; 87(2): 434 - 449.
[Full Text] [PDF]

Home page
 Ann Rheum DisHome page
H K Chang, W C Jang, S B Park, S M Han, Y H Nam, S S Lee, J U Kim, and H S Lee
Association between interleukin 6 gene polymorphisms and Behcet's disease in Korean people
Ann Rheum Dis, February 1, 2005; 64(2): 339 - 340.
[Full Text] [PDF]

Home page
 BloodHome page
J. Cavet, A. M. Dickinson, J. Norden, P. R. A. Taylor, G. H. Jackson, and P. G. Middleton
Interferon-{gamma} and interleukin-6 gene polymorphisms associate with graft-versus-host disease in HLA-matched sibling bone marrow transplantation
Blood, September 1, 2001; 98(5): 1594 - 1600.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
A. Crilly, N. Maiden, H. A Capell, and R. Madhok
Predictive value of interleukin 1 gene polymorphisms for surgery
Ann Rheum Dis, September 1, 2000; 59(9): 695 - 699.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.