Skip Navigation

This Article
Right arrow Print PDF (3006K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Gimmi, E. R.
Right arrow Articles by Deckman, I. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gimmi, E. R.
Right arrow Articles by Deckman, I. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 17 6983-6998
© 1989

MOLECULAR BIOLOGY

Alterations in the pre-mRNA topology of the bovine growth hormone polyadenylation region decrease poly(A) site efficiency

Edward R. Gimmi1,2,+, Mitchell E. Reff1,2,+ and Ingrid C. Deckman2,*

1Department of Microbiology and Immunology, Temple University of School of Medicine Philadelphia, PA 19140, USA 2Department of Molecular Genetics, Smith Kline and French Laboratories 709, Swedeland Road, King of Prussia, PA 19406, USA

*To whom correspondence should be addressed

Received May 10, 1989. Revised July 31, 1989. Accepted July 31, 1989.

RNase mapping experiments show that the bovine growth hormone (bGH) poly(A) region forms an extensive hairpin loop. Mutants were prepared to change poly(A) region pre-mRNA structure and cleavage site efficiency without altering necessary sequences. An inverted repeat which includes the poly(A) cleavage site was created by insertion of a linker upstream of the poly(A) region to compete with any wild-type secondary structure. RNA mapping analyses show alterations in the nuclease accessibility of this mutant at the natural site of cleavage. This mutant shows a 75% drop in relative reporter gene expression at the steady-state protein and RNA levels. When the linker is inserted as a direct repeat, expression is equivalent to wildtype levels. To show that transcription was not terminated by the inverted repeat, the SV40 late poly(A) region was inserted downstream. These mutants show restored expression and processing at the downstream site. Our experiments reveal that the conformation of the poly(A) site pre-mRNA is important in mediating efficient cleavage polyadenylation.

+Present address: The Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
G. M. Gilmartin
Eukaryotic mRNA 3' processing: a common means to different ends
Genes & Dev., November 1, 2005; 19(21): 2517 - 2521.
[Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H. Hans and J. C. Alwine
Functionally Significant Secondary Structure of the Simian Virus 40 Late Polyadenylation Signal
Mol. Cell. Biol., April 15, 2000; 20(8): 2926 - 2932.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
L. C. Chao, A. Jamil, S. J. Kim, L. Huang, and H. G. Martinson
Assembly of the Cleavage and Polyadenylation Apparatus Requires About 10 Seconds In Vivo and Is Faster for Strong than for Weak Poly(A) Sites
Mol. Cell. Biol., August 1, 1999; 19(8): 5588 - 5600.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. T. Das, B. Klaver, and B. Berkhout
A Hairpin Structure in the R Region of the Human Immunodeficiency Virus Type 1 RNA Genome Is Instrumental in Polyadenylation Site Selection
J. Virol., January 1, 1999; 73(1): 81 - 91.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. R. Graveley, E. S. Fleming, and G. M. Gilmartin
Restoration of Both Structure and Function to a Defective Poly(A) Site by in Vitro Selection
J. Biol. Chem., December 27, 1996; 271(52): 33654 - 33663.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.