Nucleic Acids Research, 1989, Vol. 17, No. 17 7013-7028
© 1989
MOLECULAR BIOLOGY |
Structure of a mouse erythroid 5-aminolevulinate synthase gene and mapping of erythroid-specific DNAse I hypersensitive sites
The Wistar Institute 3601, Spruce Street, Philadelphia, PA 19104, USA
*To whom correspondence should be addressed
Received March 16, 1989. Revised July 21, 1989. Accepted July 21, 1989.
The enzyme 5-aminolevulinate synthase (ALA-S) catalyzes the first step in heme biosynthesis. In this study, the mouse erythroid gene has been cloned and analyzed in order to investigate the regulation of ALA-S expression during erythroid differentiation. The gene spans {small tilde}24 kbp and consists of 11 exons and 10 introns. The first exon is 37 bp, non-coding, and followed by a 6 kb intron. The mRNA capsite was mapped by primer extension and defines a promoter that contains no apparent TATA element. S1 nuclease analysis detects the presence at low levels of a 45 bp-deleted form of the ALA-S mRNA created by the use of an alternative splice site at the intron 2/exon 3 junction. Five DNAse I hypersensitive sites were detected in chromatin from uninduced and induced MEL cells. One site is at the promoter; the others are in the body of the gene. No significant differences were observed in the patterns or intensity of the hypersensitive sites in the uninduced and induced MEL cells, however, no sites in ALA-S were observed in NIH 3T3 cells or in deproteinized DNA. Thus, these sites are specific for erythroid chromatin but appear to be established at an earlier stage of differentiation than represented by the uninduced MEL cell.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Podvinec, C. Handschin, R. Looser, and U. A. Meyer Identification of the xenosensors regulating human 5-aminolevulinate synthase PNAS, June 15, 2004; 101(24): 9127 - 9132. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C. Brown, S. Pattison, J. van Ree, E. Coghill, A. Perkins, S. M. Jane, and J. M. Cunningham Distinct Domains of Erythroid Kruppel-Like Factor Modulate Chromatin Remodeling and Transactivation at the Endogenous {beta}-Globin Gene Promoter Mol. Cell. Biol., January 1, 2002; 22(1): 161 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Dzikaite, A. Kanopka, J. H. Brock, A. Kazlauskas, and O. Melefors A novel endoproteolytic processing activity in mitochondria of erythroid cells and the role in heme synthesis Blood, July 15, 2000; 96(2): 740 - 746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. R. de Mena, M. A. Fernandez-Moreno, B. Bornstein, L. S. Kaguni, and R. Garesse Structure and Regulated Expression of the delta -Aminolevulinate Synthase Gene from Drosophila melanogaster J. Biol. Chem., December 24, 1999; 274(52): 37321 - 37328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. H. Surinya, T. C. Cox, and B. K. May Identification and Characterization of a Conserved Erythroid-specific Enhancer Located in Intron 8 of the Human 5-Aminolevulinate Synthase 2 Gene J. Biol. Chem., July 3, 1998; 273(27): 16798 - 16809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. H. Surinya, T. C. Cox, and B. K. May Transcriptional Regulation of the Human Erythroid 5-Aminolevulinate Synthase Gene. IDENTIFICATION OF PROMOTER ELEMENTS AND ROLE OF REGULATORY PROTEINS J. Biol. Chem., October 17, 1997; 272(42): 26585 - 26594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P Ponka Tissue-specific regulation of iron metabolism and heme synthesis: distinct control mechanisms in erythroid cells Blood, January 1, 1997; 89(1): 1 - 25. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Lathrop and M. Timko Regulation by heme of mitochondrial protein transport through a conserved amino acid motif Science, January 22, 1993; 259(5094): 522 - 525. [Abstract] [PDF]
|
|