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Nucleic Acids Research, 1989, Vol. 17, No. 17 7029-7043
© 1989

GENOME STRUCTURE AND MAPPING

Cloning of the complete Mycoplasma pneumoniae genome

Rainer Wenzel and Richard Herrmann

Department of Microbiology, University of Heidelberg Im Neuenheimer Feld 282, D-6900 Heidelberg, FRG

Received July 25, 1989. Accepted August 11, 1989.

The complete genome of Mycoplasma pneumoniae was cloned in an ordered library consisting of 34 overlapping or adjacent cosmids, one plasmid and two {lambda} phages. The genome size was determined by adding up the sizes of either the individual unique EcoRI restriction fragments of the gene bank or of the XhoI fragments of genomic M. pneumoniae DNA. The values from these calculations, 835 and 849 kbp, are in good agreement. An XhoI restriction map was constructed by identifying adjacent DNA fragments by probing with selected cosmid clones.


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