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Nucleic Acids Research, 1989, Vol. 17, No. 18 7149-7158
© 1989

CHEMISTRY

Universal solid supports for the synthesis of oligonucleotides with terminal 3'-phosphates

W. T. Markiewicz1 and T. K. Wyrzykiewicz

Institute of Bioorganic Chemistry, Polish Academy of Sciences Noskowskiego 12/14, 61–704 Poznan, Poland

1To whom correspondence should be addressed

Received August 14, 1989. Accepted October 21, 1989.

The preparation of two types of supports based on controlled pore glass (CPG) is presented. These supports are compatible with established phosphoroamidite chemistry of oligonucleotides synthesis giving rise to an oligonucleotide with terminal 3'-phosphate function during final deprotection. CPG was modified with: (i) methacrylic acid derivatives and 2-mercaptoethanol (1) or (ii) aminoalkylsilane, succinic anhydride and benzidine (2). Support 2 can be also used for the synthesis of partially protected oligonucleotide 3'-phosphates


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