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Nucleic Acids Research, 1989, Vol. 17, No. 18 7159-7165
© 1989

MOLECULAR BIOLOGY

The selenocysteine-inserting opal suppressor serine tRNA from E.coli is highly unusual in structure and modification

Astrid Schön, August Böck1, Günther Ott2 and Dieter Söll*,

Department of Molecular Biophysics and Biochemistry, Yale University New Haven, CT 06511, USA 1Institut für Genetik und Mikrobiologie, Universität München 8000 Munich 19, FRG 2Laboratorium für Biochemic, Universität Bayreuth 8580 Bayreuth, FRG

*To whom correspondence should be addressed

Received August 11, 1989. Accepted August 21, 1989.

Selenocysteine is cotranslationally incorporated into selenoproteins in a unique pathway involving tRNA mediated suppression of a UGA nonsense codon (1–3). The DNA sequence of the gene for this suppressor tRNA from Escherichia coli predicts unusual features of the gene product (4). We determined the sequence of this serine tRNA (tRNAUCASer) It is the longest tRNA (95 nt) known to date with an acceptor stem of 8 base pairs ancf lacks some of the ‘invariant’ nucleotides found in other tRNAs. It is the first E. coli tRNA that contains the hypermodified nucleotide i6 adjacent to the UGA-recognizing anticodon UCA. The implications of the unusual structure and modification of this tRNA on recognition by seryl-tRNA synthetase, by tRNA modifying enzymes, and on codon recognition are discussed


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