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Nucleic Acids Research, 1989, Vol. 17, No. 18 7203-7209
© 1989

MOLECULAR BIOLOGY

Nuclear and cytoplasmic sites for anti-sense control

Marc Cornelissen

Plant Genetic Systems NV, J. Plateaustraat 22, B9000 Gent, Belgium

Received July 18, 1989. Accepted August 24, 1989.

In transgenic tobacco, anti-sense control of the chimaeric bar (bialophos resistance) gene is manifested by a reduced bar transcript level and a reduced synthesis of bar translational product (PAT, phosphinothricin acelyl transferase) per transcript (1). This study shows that the anti-bar gene controls the transcript level and the translation efficiency of the bar mRNA through independent events and at different cellular sites. On the basis of these results a model for the mechanism of anti-sense control is proposed


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