Nucleic Acids Research, 1989, Vol. 17, No. 18 7263-7272
© 1989
MOLECULAR BIOLOGY |
Sequence microheterogeneity is generated at junctions of programmed DNA deletions in Tetrahymena thermophila
Division of Basic Sciences, Fred Hutchinson Cancer Research Center 1124 Columbia Street, Seattle, WA, 98104, USA
Received June 20, 1989. Revised August 17, 1989. Accepted August 17, 1989.
Regulated DNA deletions are known to occur to thousands of specific DNA segments in Tetrahymena during, macronuclear development. In this study we determined the precision of this event by examining the junction sequences produced by three different deletions in many independent catyonidal lines. 0.9 kb deletions in region M produce at least 3 types of junction sequences, of which two have been determined and found to be different by 4 bp. The alternative 0.6 kb deletions in this region are much less variable. 1.1 kb deletions in region R, known from a previous study to be slightly variable, produce two types of junction sequences which are different from each other by 3 bp. Thus, developmentally regulated deletions in Tetrahymena can produce sequence microheterogeneity at their junctions. This process contribetes significantly to the diversification of Tetrahymena's somatic genome
*Department of Biology, Creighton University, Omaha, NE 68178
+Department of Microbiology, Health Sciences Center, SUNY at Stony Brook, Stony Brook, NY 11794, USA
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