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Nucleic Acids Research, 1989, Vol. 17, No. 18 7283-7294
© 1989

MOLECULAR BIOLOGY

Similarity of minus origins of replication and flanking open reading frames of plasmids pUB110, pTB913 and pMV158

Danië van der Lelie+, Sierd Bron*, Gerard Venema and Linda Oskam

Department of Molecular Genetics, Biological Centre, University of Gromngen Kerklaan 30, 9751 NN Haren, The Netherlands

*To whom correspondence should be addressed

+Present address: TransgÈne, 11 rue de Molsheim, Strasbourg, France

Received June 13, 1989. Revised August 21, 1989. Accepted August 21, 1989.

Plasmids pMV 158 and pTB913, originating from Streptococcus agalactiae and a thermophilic Bacillus respectively, were sequenced to completion. Both contained a BA3-type minus origin of replication and an RSA-site, believed to constitute a site-specific recombination site. These two regions were more than 99% homologous to the corresponding regions of the Staphylococcus aureus plasmid pUB110. Deleting the BA3-type minus origin resulted in the accumulation of a considerable amount of single-stranded DNA, both in L.lactis subsp. lactis and B.subtilis, indicating that this minus origin was functional in both bacterial species. Like pUB110, both plasmids contained an open reading frame encoding a putative plasmid recombinalion enzyme (Pre protein), which was located downstream of the RSA-site. On the basis of sequence comparisons between pUB110, pMV158, pTB913, pT181, pE194, pNE131 and pT48 two distinct families of RSA-sites and Pre proteins could be distinguished


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