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Nucleic Acids Research, 1989, Vol. 17, No. 18 7303-7314
© 1989

MOLECULAR BIOLOGY

Isolation and characterization of the Drosophila translational elongation factor 2 gene

Yevgenya Grinblat1, Nicholas H. Brown1 and Fotis C. Kafatos1,2

1The Biological Laboratones, Harvard University 16 Divinity Avenue, Cambridge, MA 02138, USA 2Institute of Molecular Biology and Biotechnology, Research Center of Crete and Department of Biology, University of Crete Heraklion, 711 10 Crete, Greece

Received June 10, 1989. Revised July 18, 1989. Accepted August 16, 1989.

We have isolated a cDNA clone that encodes the Drosophila melanogaster elongation factor 2 (EF2), a protein involved in the elongation step of protein synthesis. This identification was based on the high degree of its amino acid sequence identity (greater than 80%) to that of hamster EF2. The gene encoding Drosophila EF2 is found at position 39E-F of the 2L chromosomal arm and may be identical to the M(2)H locus, which produces a Minute phenotype when mutated. The genomic organization of the locus includes four exons. Conserved sequence segements shared with a variety of GTP binding proteins are found in the amino terminal third of the protein, and segments unique to EF2 and its prokaiyotic functional homolog, EF-G, are in the carboxy terminal half; these two regions are segregated in two respective exons


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