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Nucleic Acids Research, 1989, Vol. 17, No. 19 7671-7680
© 1989

MOLECULAR BIOLOGY

The c1 genes of P1 and P7

Francis A. Osborne, Sonja R. Stovall and Barbara R. Baumstark*

Department of Biology, Georgia State University Atlanta, GA 30303, USA

*To whom correspondence should be addressed

Received July 11, 1989. Revised August 29, 1989. Accepted August 29, 1989.

The cl genes of the heteroimmune phages P1 and P7 were sequenced and their products were compared. P7cl expression was correlated with the translation in vitro of a protein whose predicted molecular weight (33,000 daltons) is indistinguishable from that of the P1cl repressor. The cl regions from both P1 and P7 were found to contain open reading frames capable of coding for a 283-amino acid protein whose predicted secondary structure lacks the helix-turn-helix motif commonly associated with repressor proteins. Two P1cl amber mutations were localized to the 283-amino acid open reading frame. The P1cl and P7cl sequences were found to differ at only 18 positions, all but two of which alter the third position of the affected codon and do not alter the amino acid sequence of the protein. Plasmids expressing the cl gene from either phage cause the repression of transcription from a cloned promoter situated upstream of P1cl.


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