Nucleic Acids Research, 1989, Vol. 17, No. 19 7879-7889
© 1989
ENZYMOLOGY |
Catalytic activity is retained in the Tetrahymena group I intron despite removal of the large extension of element P5
The Salk Institute for Biological Studies PO Box 85800, San Diego, CA 92138, USA
+Present address: Department of Molecular Biology, Research Institute of Scripps Clinic, 10666 N. Torrey Pines Road, La Jolla, CA 92037, USA
*To whom correspondence should be addressed
Received June 30, 1989. Revised August 23, 1989. Accepted August 23, 1989.
We have made sizeable internal deletions within the self-splicing group I intron of Tetrahymena thermophila. Deletions were made in a piecewise manner In order to remove secondary structural elements thought to be extraneous to the catalytic center of the molecule. The resulting deletion mutants retain self-splicing activity, albeit under modified reaction conditions that enhance duplex stability. Considering those portions of the molecule that can be deleted without a loss of catalytic activity, one is left with a catalytic center of approximately 130 nucleotides that is solely responsible for the molecule's activity.
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