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Nucleic Acids Research, 1989, Vol. 17, No. 19 7923-7944
© 1989

MOLECULAR BIOLOGY

Non-contiguous regions of Z-DNA in a DNA dodecamer

Nhi Hua, Gijs A.van der Marel1, Jacques H.van Boom1 and Juli Feigon*,

Department of Chemistry & Biochemistry and Molecular Biology Institute, University of California Los Angeles, CA 90024-1569, USA 1Gorlaeus Laboratories, University of Leiden 2300 RA Leiden, The Netherlands

*To whom correspondence should be addressed

Received April 25, 1989. Revised August 28, 1989. Accepted August 28, 1989.

The conformation of the self-complimentary DNA dodecamer d(br5CGbr5CGAATTbr5CGbr5CG) has been investigated in a variety of salt and solvent conditions by one and two-dimensional 1H NMR. In low salt aqueous solutions, the molecule forms a regular B-DNA structure similar to the unmodified dodecamer. However, in aqueous solution containing high salt concentration and methanol, the dodecamer adopts a structure in which the br5CGbr5CG ends of the molecule are in a Z-DNA like conformation and the AATT region is neither standard B-DNA nor Z-DNA. The implications of these results for the structure of junctions between B and Z-DNA and the sequence specificity of Z-DNA are discussed.


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