Skip Navigation

This Article
Right arrow Print PDF (4222K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kawamoto, T.
Right arrow Articles by Kakunaga, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kawamoto, T.
Right arrow Articles by Kakunaga, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 2 523-537
© 1989

MOLECULAR BIOLOGY

DNA bending and binding factors of the human ß-actin promoter

Takeshi Kawamoto, Kozo Makino1, Satoshi Orita, Atsuo Nakata1 and Takeo Kakunaga

Departments of Oncogene Research 3–1 Yamadaoka, Suita, Osaka 565, Japan 1Experimental Chemotherapy, Research Institute for Microbial Disease, Osaka University 3–1 Yamadaoka, Suita, Osaka 565, Japan

Received November 9, 1988. Revised December 22, 1988. Accepted December 22, 1988.

Transcription of the B -actin gene is rapidly inducible in response to serum stimulation. To determine the regions responsible for serum inducible and basal level expression, the human B -actin promoter was subjected to mutational analysis-Two distinct elements, the CCAAT homology and the ß-actin specific conserved sequences, were found by a chloramphenicol acetyl trans ferase expression assay and sequence comparisons, and then analyzed for possible functions. Using a DNA bend assay, it was shown that the conserved sequences included the core of a sequence-directed bend of DNA. Gel mobility shift and DNase I protection assays revealed that the conserved sequences and the CCAAT homology were recognized by binding factors in HeLa cell extracts.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J.-i. Nishikawa, M. Amano, Y. Fukue, S. Tanaka, H. Kishi, Y. Hirota, K. Yoda, and T. Ohyama
Left-handedly curved DNA regulates accessibility to cis-DNA elements in chromatin
Nucleic Acids Res., November 15, 2003; 31(22): 6651 - 6662.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Ohyama and T. Ohyama
Bent DNA in the Human Adenovirus Type 2E1A Enhancer Is an Architectural Element for Transcription Stimulation
J. Biol. Chem., November 1, 1996; 271(44): 27823 - 27828.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Filatov and L. Thelander
Role of a Proximal NF-Y Binding Promoter Element in S Phase-specific Expression of Mouse Ribonucleotide Reductase R2 Gene
J. Biol. Chem., October 20, 1995; 270(42): 25239 - 25243.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. C. Bash, J. M. Vargason, S. Cornejo, P. S. Ho, and D. Lohr
Intrinsically Bent DNA in the Promoter Regions of the Yeast GAL1-10 and GAL80 Genes
J. Biol. Chem., January 5, 2001; 276(2): 861 - 866.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.