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Nucleic Acids Research, 1989, Vol. 17, No. 2 617-629
© 1989

CHEMISTRY

Complex structural behavior of oligopurine-ligopyrimidine sequence cloned within the supercoiled plasmid

Pawel Parniewski, Grazyna Galazka, Andrzej Wilk and Jan Klysilc*

Centre of Molecular and Macromolecular Studies, Department of Bioorganic Chemistry, Polish Academy of Sciences 90-362 Lodz, Boczna 5, Poland

*To Whom correspondence should be addressed

Received October 10, 1988. Revised December 1, 1988. Accepted December 23, 1988.

Synthetic sequence GATCC(AG)7ATCG(AT)4CG(AG)7 was cloned into plasmid and its structural behavior under the infuence of supercoiling was analysed by chemical modification at variety of experimental conditions. It was found that this sequence adopts at least two different non-B conformations depending on –{sigma} and pH values. Moreover, 12 nucleotide long non-purepyr spacer region separating two identical (AG)7 blocks does not provide a significant energy barrier protecting against unusual structures formation.


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