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Nucleic Acids Research, 1989, Vol. 17, No. 20 8033-8045
© 1989

MOLECULAR BIOLOGY

Molecular organization of sbcC, a gene that affects genetic recombination and the viability of DNA palindromes in Escherichia coli K-12

Isam S. Naom, Stuart J. Morton, David R.F. Leach1 and Robert G. Lloyd

Department of Genetics, University of Nottingham, Queens Medical Centre Nottingham NG7 2UH, UK 1Department of Molecular Biology, University of Edinburgh King's Buildings, Edinburgh EH9 3JR, UK

Received August 11, 1989. Revised September 12, 1989. Accepted September 12, 1989.

The sbcC gene product of Escherichia coli interferes with the growth of a {lambda}red gam phage carrying a long palindrome in its DNA. This phenotype was used to identify recombinant plasmids harbouring the wild-type gene and to isolate sbcC mutant derivatives carrying Tn1000 insertions. Analysis of these plasmids located sbcC between proC and phoR at a slightly different position from that reported before (Lloyd, R. G. and Buckman, C. 1985, J. Bacteriol. 164, 836–844). Nucleotide sequencing revealed that the gene spans a DNA segment of 3.3 kb that encodes a poorly expressed protein of 118 kDa and which lies downstream of a gene of unknown function that encodes a polypeptide of 45 kDa. The amino acid sequence of SbcC contains a nucleotide binding fold similar to that in RecB and other recombination proteins.


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