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Nucleic Acids Research, 1989, Vol. 17, No. 20 8273-8282
© 1989

MOLECULAR BIOLOGY

Sequence-directed curvature of repetitive AluI DNA in constitutive heterochromatin of Artemia franciscana

Robert Benfante, Nicoletta Landsberger, Grazia Tubiello and Gianfranco Badaracco*

Dipartimento di Genetica e di Biologia dei Microrganismi, Universitá di Milano Via Celoria 26, 20133 Milan, Italy

*To whom correspondence should be addressed

Received July 24, 1989. Revised September 14, 1989. Accepted September 14, 1989.

An Alu I family of repeated DNA sequence 113 bp in length was found to be the major component of the heterochromatin in Artemia franciscana. On the basis of the analysis of cloned oligomeric (monomer to examer) heterochromatic fragments we predicted that the sequence could produce a stable curvature in chromosomal DNA. This prediction was confirmed by polyacrylamide gel electrophoresls analysis and by electron microscope observations. The anomalous mobility of these fragments is reversed when the DNA samples are electrophoresed in the presence of distamycin A. Moreover treatment of living Artemia with this drug produces visible decondensatlon of heterochromatic masses in the interphase nuclei.


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M. C. Motta, N. Landsberger, C. Merli, and G. Badaracco
In Vitro Reconstitution of Artemia Satellite Chromatin
J. Biol. Chem., July 17, 1998; 273(29): 18028 - 18039.
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