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Nucleic Acids Research, 1989, Vol. 17, No. 21 8553-8567
© 1989

MOLECULAR BIOLOGY

Sequence, structure and evolution of the gene coding for sn-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster

Glenn C. Bewley, Julia L. Cook*, Shinichi Kusakabe2, Terumi Mukai2, Donna L. Rigby1 and Geoff K. Chambers1,+

Department of Genetics, North Carolina State University Raleigh, North Carolina 27695-7614, USA 1Museum of Comparative Zoology, Harvard University Cambridge, MA 02138, USA 2Department of Biology, Kyushu University Fukuoka 812, Japan

Received July 26, 1989. Revised September 27, 1989. Accepted September 27, 1989.

We present the complete nucleotide and deduced amino acid sequence for the gene encoding Drosophila sn-glycerol-3-phospnate dehydrogenase. A transcription unit of 5kb was identified which is composed of eight protein encoding exons. Three classes of transcripts were shown to differ only in the 3'-end and to code for three protein isoforms each with a different C-terminal amino acid sequence. Each transcript is shown to arise through the differential expression of three isotype-specific exons at the 3'-end of the gene by a developmentally regulated process of 3'-end formation and alternate splicing pathways of the pre-mRNA. In contrast, the 5'-end of the gene is simple in structure and each mRNA is transcribed from the same promoter sequence. A comparison of the organization of the Drosophila and murine genes and the primary amino acid sequence between a total of four species indicates that the GPDH gene-enzyme system is highly conserved and is evolving slowly.

*Present address: Ochsner Medical Foundation, Laboratory of Molecular Genetics, New Orleans, LA 70121, USA

+Present address: Department of Biochemistry, Victoria University of Wellington, Wellington, New Zealand


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