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Nucleic Acids Research, 1989, Vol. 17, No. 21 8683-8694
© 1989

MOLECULAR BIOLOGY

Cooperativity in nucleosomes assembly on supercoiled pBR322 DNA

Piero Forte1, Luisa Leoni, Beatrice Sampaolese and Maria Savino1

Centro di Studio per gli Acidi Nucleici del CNR Rome, Itlay 1Dipartimento di Genetica e Biologia Molecolare, Università di Roma ‘La Sapienza’ Rome, Italy

Received October 6, 1989. Revised October 6, 1989. Accepted October 6, 1989.

Many studies have shown that in reconstituted chromatin model systems containing only purified DNA and histone octamer, nucleosomes can adopt well defined locations with respect to DNA nucleotide sequence. Recently, nucleosome-nucleosome interactions were suggested as one of the factors underlying preferential nucleosomes positioning. In the present paper this aspect has been studied by topological analysis and electron microscopy visualization of minichromosomes reconstituted at different histone/DNA ratios. Both methods suggest that cooperativity plays a role in nucleosomes formation. A linear cooperative model in which nucleosomes are formed on discrete sites with cooperative interactions occurring only between nearest neighbours allows to calculate the cooperative constant. The reported results show that basic interactions, which are of relevance in the process of chromatin folding, are present also in very simple model system.


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