Nucleic Acids Research, 1989, Vol. 17, No. 21 8727-8739
© 1989
MOLECULAR BIOLOGY |
Duplication and transcription of procyclin genes in Trypanosoma brucei
Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie Postfach 3640, D-7500 Karlsruhe 1, UK 1Deutsches Krebsforschungszentrum, Institut för Virusforschung Heidelberg, FRG, UK 2Department of Biochemistry, University of Cambridge Cambridge, UK
*To whom correspondence should be addressed
Received September 4, 1989. Accepted October 29, 1989.
The genes encoding procyclin, the major glycoprotein expressed on the surface of procyclic forms of Trypanosoma brucei, comprise a multigene family. It has previously been demonstrated that procyclin genes in cloned trypanosome strains from Kenya and Uganda show restriction fragment polymorphisms. A detailed study of the Kenyan strain 227 has revealed that procyclin genes are arranged in tandem at 3 distinct loci (Pro A, B and C and that the polymorphism is due to the duplication of 1.3 kb in the Pro A locus, which has generated an additional procyclin gene. Northern blot analysis has shown that at least 2 loci are transcribed and that a minimum of 3 procyclin genes are expressed within a cloned line. The transcription of procyclin genes is resistant to 1mg ml–1 
-amanitin, whereas that of the 5' flanking gene in the Pro A locus is sensitive. This observation suggests that the two genes form part of separate transcription units with a promoter between them.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Utz, I. Roditi, C. Kunz Renggli, I. C. Almeida, A. Acosta-Serrano, and P. Butikofer Trypanosoma congolense Procyclins: Unmasking Cryptic Major Surface Glycoproteins in Procyclic Forms. Eukaryot. Cell, August 1, 2006; 5(8): 1430 - 1440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Webb, R. Burns, L. Ellis, N. Kimblin, and M. Carrington Developmentally regulated instability of the GPI-PLC mRNA is dependent on a short-lived protein factor Nucleic Acids Res., March 8, 2005; 33(5): 1503 - 1512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Thomson, D. J. Lamont, A. Mehlert, J. D. Barry, and M. A. J. Ferguson Partial Structure of Glutamic Acid and Alanine-rich Protein, a Major Surface Glycoprotein of the Insect Stages of Trypanosoma congolense J. Biol. Chem., December 6, 2002; 277(50): 48899 - 48904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Vassella, J. V. Den Abbeele, P. Bütikofer, C. K. Renggli, A. Furger, R. Brun, and I. Roditi A major surface glycoprotein of Trypanosoma brucei is expressed transiently during development and can be regulated post-transcriptionally by glycerol or hypoxia Genes & Dev., March 1, 2000; 14(5): 615 - 626. [Abstract] [Full Text]
|
|
|
|
 |
|
 N. R. Sturm, M. C. Yu, and D. A. Campbell Transcription Termination and 3'-End Processing of the Spliced Leader RNA in Kinetoplastids Mol. Cell. Biol., February 1, 1999; 19(2): 1595 - 1604. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Butikofer, E Vassella, S Ruepp, M Boschung, G Civenni, T Seebeck, A Hemphill, N Mookherjee, T. Pearson, and I Roditi Phosphorylation of a major GPI-anchored surface protein of Trypanosoma brucei during transport to the plasma membrane J. Cell Sci., January 6, 1999; 112(11): 1785 - 1795. [Abstract] [PDF]
|
|
|
|
 |
|
 I. Chaves, J. Zomerdijk, A. Dirks-Mulder, R. W. Dirks, A. K. Raap, and P. Borst Subnuclear localization of the active variant surface glycoprotein gene expression site in Trypanosoma brucei PNAS, October 13, 1998; 95(21): 12328 - 12333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ruepp, A. Furger, U. Kurath, C. K. Renggli, A. Hemphill, R. Brun, and I. Roditi Survival of Trypanosoma brucei in the Tsetse Fly Is Enhanced by the Expression of Specific Forms of Procyclin J. Cell Biol., June 16, 1997; 137(6): 1369 - 1379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E Wirtz and C Clayton Inducible gene expression in trypanosomes mediated by a prokaryotic repressor Science, May 26, 1995; 268(5214): 1179 - 1183. [Abstract] [PDF]
|
|