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Corrigendum for Hemsley et al., Nucl. Acids Res. 17 (16) 6545-6551.
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Nucleic Acids Research, 1989, Vol. 17, No. 21 8915
© 1989

Corrigendum

A simple method for site-directed mutagenesis using the polymerase chain reaction

A. Hemsley, N. Arnheim, M.D. Toney, G. Cortopassi and D.J. Galas

Incorrect details regarding PCR conditions were supplied for the Materials and Methods section of this paper. The correct details are published below.

PCR conditions

Template DNA (10 fmol) and primer sets (1 µM each) were incubated in a Perkin Elmer Cetus Thermal Cycler in 100 µl reaction volumes containing 10 mM Tris-HCl pH 8.3, 50 mM KCl, 2.5 mM MgCl2, 10 µg gelatin, 0.2 mM of each of NTP and 2 units Taq polymerase (5).


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