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Nucleic Acids Research, 1989, Vol. 17, No. 22 8979-8989
© 1989

CHEMISTRY

Synthesis of N2, N2, 7-trimethylguanosine cap derivatives

R. Iwase, M. Sekine1,*, Y. Tokumoto2, Y. Ohshima2 and T. Hata

Department of Life Chemistiy, Tokyo Institute of Technology Nagatsuta, Midoriku, Yokohama 227, Japan 1Department of Environmental Chemistiy and Engineering, Tokyo Institute of Technology Midoriku, Yokohama 227, Japan 2Department of Biology, Faculty of Science, Kyushu University Higashiku, Fukuoka 812, Japan

*To whom correspondence should be addressed

Received September 7, 1989. Accepted October 6, 1989.

Several derivatives of N2,N2-7-trimethylguanosine (m32,2,7G)-cap, which was found at the 5' ends of small nuclear RNAs, were synthesized by use of S-phenyl N2,N2,7-trimethylguanosine 5'-phosphorothioate (PhSpm32,2,7G) as a key intermediate. This compound was activated by iodine in the presence of phosphoric acid and diphosphoric acid to give N2,N27-trimethylguanosine-5'diphosphate (ppm32,2,7G) and 5'-triphosphate (pppm32,2,7G), respectively. Similar reactions of PhSpm32,2,7G with ADP and GDP gave capped dinucleoside triphosphates, m32,2,7G5' pppA and m32,2,7G5' pppG, respectively.


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[Abstract] [PDF]


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